Journal: Annals of Biomedical Engineering

Article Title: Harnessing the Electrochemical Effects of Electroporation-Based Therapies to Enhance Anti-tumor Immune Responses

doi: 10.1007/s10439-023-03403-x

Figure Lengend Snippet: Viability and activation of THP-1 macrophages are affected by culture medium pH. a Effect of increasing pH on THP-1 macrophage CD14 expression detected in viable THP-1 cells using flow cytometry analysis. p = 0.1678 (ns). b Effect of increasing pH on THP-1 macrophage cell viability. * p < 0.01 versus all other pH levels tested, n = 6 independent experiments. c Effect of increasing pH on THP-1 macrophage CD206 expression (M2 activation marker) detected in viable THP-1 cells using flow cytometry analysis. ** p < 0.0001 versus pH 7.5 and pH 6.5, * p < 0.01 versus pH 6.5, n = 6 independent experiments. d Effect of increasing pH on THP-1 macrophage CD80 expression (M1 activation marker) detected in viable THP-1 cells using flow cytometry analysis. * p < 0.0001 versus pH 7.5 and pH 6.5, # p = 0.9094 (ns) versus pH 6.5, n = 6 independent experiments.

Article Snippet: After 48 hour exposure to altered pH conditions, THP-1 macrophages were labeled in 100 μL/million cells in eBioscience™ Flow Cytometry Staining Buffer (Thermo Fisher) with 0.1 μL Zombie Aqua™ (BioLegend, San Diego, CA), 2 μL Brilliant Violet 421™ anti-CD-80 antibody (BioLegend, Cat# 305222), 1 μL APC anti-CD-206 (MMR) antibody (BioLegend Cat# 321110), and 5 μL FITC anti-CD-14 antibody (Abcam Cat# ab28061) prior to fixation and staining using commercial staining and fixation buffers (Thermo Fisher).

Techniques: Activation Assay, Expressing, Flow Cytometry, Marker